Characterizing the Skin and Gut Microbiome of Alopecia Areata Patients
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Keywords
alopecia areata, microbiome, dysbiosis
Abstract
Background: Alopecia areata (AA) is caused by autoimmune attack of the hair follicle. The exact pathogenesis is unknown, but hypotheses include innate immunity imbalance, environmental exposures, genetic predisposition, and possibly the microbiome. The objective of this study was to characterize the skin and gut microbiome of AA patients, and compare microbial composition to healthy individuals.
Methods: This was a pilot, case-control study. Scalp and fecal microbiome samples were collected from 25 AA patients, and 25 age, gender, and race-matched healthy controls in Southern California with no significant difference in demographic characteristics. After library preparation and identification of bacterial and fungal taxonomy, multivariant analysis was performed to compare AA and healthy microbiomes.
Results: The AA scalp microbiome was significant for decreased Clostridia and Malasseziomycetes, and the gut microbiome was significant for decreased Bacteroidia and increased Bacilli (p<0.05) compared to healthy controls.
Conclusions: The composition of the AA bacterial and fungal, scalp and gut microbiome is significantly different than healthy individuals. Future directions include using this data to characterize microbial changes associated with AA patient diet, relating to disease severity, and predicting disease progression, prognosis and/or therapeutic response.
References
2. Borde A, Astrand A. Alopecia areata and the gut-the link opens up for novel therapeutic interventions. Expert Opin Ther Targets 2018;22:503-11.
3. Rebello D, Wang E, Yen E, Lio PA, Kelly CR. Hair Growth in Two Alopecia Patients after Fecal Microbiota Transplant. ACG Case Rep J 2017;4:e107.
4. Caporaso JG, Lauber CL, Walters WA, et al. Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 2012;6:1621-4.
5. Walters W, Hyde ER, Berg-Lyons D, et al. Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys. mSystems 2016;1.
6. Looby CI, Maltz MR, Treseder KK. Belowground responses to elevation in a changing cloud forest. Ecol Evol 2016;6:1996-2009.
7. Greengenes the 16s rRNA gene database and tools. 2013. (Accessed January 28, 2019, at http://greengenes.secondgenome.com/downloads.)
8. 2018. Unite. (Accessed January 28, 2019, at https://unite.ut.ee/.)
9. QIIME2. 2019. (Accessed January 28, 2019, at https://qiime2.org/.)
10. Hayashi A, Mikami Y, Miyamoto K, et al. Intestinal Dysbiosis and Biotin Deprivation Induce Alopecia through Overgrowth of Lactobacillus murinus in Mice. Cell Rep 2017;20:1513-24.
11. Nair L, Dai Z, Christiano AM. Gut microbiota plays a role in the development of alopecia areata. J Investig Dermatol 2017;137:S112.
12. Bjerre RD, Bandier J, Skov L, Engstrand L, Johansen JD. The role of the skin microbiome in atopic dermatitis: a systematic review. Br J Dermatol 2017;177:1272-8.
13. Rangel SM, Paller AS. Bacterial colonization, overgrowth, and superinfection in atopic dermatitis. Clin Dermatol 2018;36:641-7.
14. Wollina U. Microbiome in atopic dermatitis. Clin Cosmet Investig Dermatol 2017;10:51-6.
15. Chew EG, Ho BS, Ramasamy S, et al. Comparative transcriptome profiling provides new insights into mechanisms of androgenetic alopecia progression. Br J Dermatol 2017;176:265-9.
16. Maslowski KM, Mackay CR. Diet, gut microbiota and immune responses. Nat Immunol 2011;12:5-9.
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